MicroRNA-derived Fragment Length Polymorphism Assay
نویسندگان
چکیده
منابع مشابه
MicroRNA-derived Fragment Length Polymorphism Assay
MicroRNA (miRNA) studies are experiencing a transition from basic research applications to clinical applications. However, the lack of reliable and sensitive miRNA detection methods has become a bottleneck in the process. Here, we report an absolute quantification method based on the competitive PCR amplification of specific miRNAs and synthetic RNA spike-ins in a single reaction. RNA spike-ins...
متن کاملRestriction Fragment Length Polymorphism (RFLP)
Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...
متن کاملApplications of restriction fragment length polymorphism.
The availability of a variety of restriction endonuclease enzymes that cleave deoxyribonucleic acid (DNA) at specific sites has made it possible to identify the presence of polymorphic regions in the isolated fragments. Such restriction fragment length polymorphism (RFLP) results owing to a variation in the number of tandem repeats (VNTR) of a short DNA segment. These VNTR sequences can uniquel...
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CleavaseFragment Length Polymorphism (CFLP) analysis is a convenient, accurate and highly sensitive method for the detection and localization of nucleic acid mutations. The assay is well suited for high-throughput screening and can be used to detect mutations in known and unknown nucleic acid samples. A recent improvement in the CFLP assay termed “temperature ramping” or “ramping” is reported ...
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The domesticated silkworm, Bombyx mori, is of high commercial importance as a silk producer and is also widely used for implementation of basic and applied research. It is important to understand its genome organization using molecular markers for genetic studies and for breeding purposes. In this study, a genetic linkage map using 204 amplified fragment length polymorphism (AFLP) markers was d...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2015
ISSN: 2045-2322
DOI: 10.1038/srep09356